Twenty-eight teams have entered the Co-Operative Challenge Trophy for season 2008/09.
Barrow AFC, Burscough FC, Fleetwood Town and Southport FC were the seeded teams and therefore received a bye into the Second Round.
The draw for the first round is as follows:
Nelson v Colne
Lancaster City v Darwen
Atherton Colleries v Leigh Genesis
Atherton LR v Bacup Borough
Daisy Hill v Ramsbottom United
Squires Gate v Radcliffe Borough
Padiham v Kendal Town
Holker Old Boys v AFC Fylde
Chorley v Skelmersdale United
Ashton Athletic v AFC Blackpool
Rossendale United v Clitheroe
Bamber Bridge v Marine
Ties will be played week commencing 10th November 2008.